|
KMID : 0903520090520040380
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 4 p.380 ~ p.388
|
|
|
An Enhanced ELISA for the Acaricide/Insecticide Fenazaquin
|
|
Kyung Kee-Sung
Kim Dae-Kyu
Kim Yun-Jung
Lee Eun-Young
Lee Jae-Koo
|
|
|
Abstract
|
|
|
|
To enhance the sensitivity of an ELISA developed for the acaricide/insecticide fenazaquin, the length of the spacer arm attached to the 7-position of the quinazoline ring in the hapten (H-I) used previously as an immunogen was shortened, and this modified hapten (H-IV) was then used as an immunogen. The new immunogen used to raise polyclonal antibodies was designated 3-(4-(4-tertbutylbenzyloxy) quinazolin-7-ylcarbamoyl)propanoic acid. Under optimized conditions of a competitive indirect ELISA (ciELISA), the former IC50 value of 96.97 ng/mL was reduced to 42.13 ng/mL, and the detection range was 10.96-161.91 ng/mL, the lowest detection limit being 1.8 ng/mL. HPLC was run to compare with and to validate the ELISA with fortified fruit and water samples; the correlation was 0.997-1.000 (n=4-5) with a slope of 0.905-1.112. The recoveries of fenazaquin from fortified samples by the ELISA ranged from 90.61 to 115.12%, which is acceptable as an ELISA for residue analysis.
|
|
|
KEYWORD
|
|
|
acaricide/insecticide, ELISA, fenazaquin, polyclonal antibody, residue analysis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|